Uburyo Ikoranabuhanga rya Recombinant Rihindura Ishusho ya PDRN

Mu myaka ibarirwa muri za mirongo, PDRN yishingikirizaga ku gukuramo utunyangingo tw’imyororokere twa salmon. Iyi nzira gakondo irangwa n’ihindagurika ry’itangwa ry’amafi, uruhererekane rwa ADN rutunguranye, n’imbogamizi mu kugenzura ubuziranenge bw’amafi—bigatuma habaho ubwumvikane burambye, kwaguka, no kubahiriza amategeko bigorana kwemeza.

IbyacuPDRN ivanzeyateguwe kugira ngo itsinde izi mbogamizi z’imiterere y’ibinyabuzima binyuze mu buhanga buhanitse bw’ibinyabuzima.

Nta nyamaswa zikomokaho, zubakiye ku buryo bwo gukora biosynthesis bugenzurwa
Hakoreshejwe E. coli DH5α nk'urubuga rw'ibinyabuzima rukora, uburyo bwihariye bwa PDRN bushyirwa mu bikorwa binyuze mu buryo bw'uturemangingo tw’ingirabuzimafatizo kandi bigakorwa neza binyuze mu gushyushya mikorobe.
Ubu buryo bukuraho kwishingikiriza ku bikoresho bikomoka ku mafi, bukemura ibibazo byo kudahuza kw'ibicuruzwa n'umutekano w'inyamaswa aho bikomoka, ariko bugahuza n'amahame agenga cyane mu masoko y'ibihugu by'Uburayi, Amerika n'isi.
Muri icyo gihe, ibicuruzwa bigumahoishingiye kuri ADN kandi ikaba yakozwe mu buryo bw'umwimerere, bigatuma bibaibikomoka ku bimera gusa, bitari inyamaswa, ariko byemewe mu buryo bw'ibinyabuzimakuri PDRN ikomoka ku salmon gakondo.

Urutonde rwateguwe neza, ntabwo ari ugukuramo ibintu mu buryo butunguranye
Bitandukanye na PDRN isanzwe iboneka binyuze mu gukuramo ibintu bidatoranyije, ikoranabuhanga rya recombinant ritumakugenzura byimazeyo uruhererekane rwa ADN n'uburebure bw'ibice.

Imiterere y'imirongo migufi ishobora gukorerwa mu rwego rwo kurwanya ububyimbirwe

Imiterere y'urunigi ruciriritse kugeza kure ishobora guhindurwa kugira ngo ifashe mu kuvugurura collagen no gusana uruhu

Iri hinduka—kuva ku gukuramo ibintu mu buryo butunguranye kugera ku gukora biosynthesis igamije—rifungura amahirwe mashya yo guteza imbere imikorere n'uburyo bwo gukora ibintu byihariye.

Uburyo bwo kwaguka no kongera umusaruro mu rwego rw'inganda
Binyuze mu guhuza uburyo bwo guhindura ubushyuhe n'ingufu no gutegura neza uturemangingo, uburyo bwo gufata plasmide no kuyibyaza umusaruro birarushaho kuba byiza.
Iyo bihujwe no gukata imiterere y'umubiri mu buryo bw'intambwe nyinshi no gutunganya chromatographic, inzira ihora igera ku ntego zayo.ubuziranenge bw'ibipimo by'ubuvuzi (≥99.5%).
Ibipimo ngenderwaho byo guhinga birushaho gutuma habaho iterambere rirambye kuva ku musaruro w’icyitegererezo kugeza ku nganda z’ubucuruzi.

Ingufu zemejwe n'amakuru abanziriza ubuvuzi
Ubushakashatsi bwakozwe mbere y'uko ubuvuzi butangira bwerekana ko PDRN ikora recombinant itangagushishikariza cyane imiterere ya kolajeni yo mu bwoko bwa I mu bantuugereranije n'ubwoko busanzwe bwa PDRN na ADN-metal complexes zikomoka kuri salmon.
Ibi bisubizo bishyigikira ikoreshwa ryayo mu gusana uruhu no kurwanya gusaza, bitanga uburyo bwoigisubizo cy'ibintu bishobora gushakisha amakuru, gishingiye ku buryo bw'imikorere.

PDRN ikoreshwa mu buryo bwa recombinant ni ikintu kirenze icyo gusimbura—ni iterambere ry’ikoranabuhanga.
Mu guhuza imiterere nyayo y'uruhererekane hamwe n'uburyo bwo gukora ibintu bugenzurwa, ikoranabuhanga rya recombinant ryongera ubushobozi bwa PDRN mu gihe ritangauburyo buhamye, butagira ingaruka ku bimera gusa, kandi busanzwekuri PDRN ikomoka ku nyamaswa—ishyiraho igipimo gishya cy’ibikoresho byo kuvugurura uruhu byo mu gisekuru gitaha.